Possible mechanisms employed by Bacillus amyloliquefaciens to generate GABA-rich rice for enhancement of collagen expression in mouse skin
碩士 === 國立中央大學 === 生醫科學與工程學系 === 107 === Abstract Skin is the largest organ in humans. Several skin conditions including wrinkles during aging could be associated with degradation of the collagen. Fibroblast is the main cell that produces collagen. Collagen type 1 (Col1) is the most abundant collagen...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | en_US |
| Published: |
2019
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/hj9445 |
| Summary: | 碩士 === 國立中央大學 === 生醫科學與工程學系 === 107 === Abstract
Skin is the largest organ in humans. Several skin conditions including wrinkles
during aging could be associated with degradation of the collagen. Fibroblast is
the main cell that produces collagen. Collagen type 1 (Col1) is the most
abundant collagen in the human body. With an aim to create a modality that can
stimulate collagen production in skin, rice was incubated with a Bacillus
amyloliquefaciens (B. amyloliquefaciens) bacteria which live in both soil and
human skin. Our results demonstrate that incubation of rice with B.
amyloliquefaciens bacteria up-regulated the level of Gamma-aminobutyric acid
(GABA). Topical application of GABA or GABA-rich rice significantly
increased the production of Col1 in mouse skin. Furthermore, in vivo knockout
of GABA receptor subunit beta-3 (GABRB3) by small interfering ribonucleic
(siRNA) markedly reduced Col1 production in the mouse skin, indicating that
GABRB3 mediates the GABA-induced Col1 production in skin.
|
|---|