The Effect of Glycerol on Skin Bacterial Fermentation and Dermal Electricity
碩士 === 國立中央大學 === 生物醫學工程研究所 === 107 === Harmful bacteria could cause human diseases. However, the diseases can result from the imbalance between the harmful and beneficial bacteria. Staphylococcus epidermidis (S. epi) and Propionibacterium acnes (P. acnes), now named as Cutibacterium acnes (C. acnes...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2019
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| Online Access: | http://ndltd.ncl.edu.tw/handle/5ga5fn |
| Summary: | 碩士 === 國立中央大學 === 生物醫學工程研究所 === 107 === Harmful bacteria could cause human diseases. However, the diseases can result from the imbalance between the harmful and beneficial bacteria. Staphylococcus epidermidis (S. epi) and Propionibacterium acnes (P. acnes), now named as Cutibacterium acnes (C. acnes) are two main bacteria in the human skin microbiome. Data from our laboratories demonstrated that both bacteria can use glycerol as a carbon source for fermentation. Electron is produced during the production of short chain fatty acids (SCDAs) from glycerol fermentation of skin bacteria. An electronic skin patch was fabricated in our laboratory to detect the electron from mouse and human skin. The studies here demonstrate that S. epi can mediate glycerol fermentation to produce higher level of electricity than P. acnes in skin of ICR mice.
In vitro data illustrated that electricity was produced to the highest level 10 min after glycerol fermentation of S. epi and declined to a baseline 60 min after. To test whether electron can function as an antioxidant to reduce free radicals induced by ultraviolet (UV), skin of ICR mice were topically applied with S. epi with glycerol for 10 and 60 min. The lipid peroxidation derived 4-hydroxynonenal (4-HNE) was used as a biomarker for the production of UV-induced free radicals. Our western blot results here demonstrate that the production of UV-induced 4-HNE was detectable when mouse skin was exposed to the culture of S. epi plus glycerol for 60 min. However, the production of UV-induced 4-HNE was dramatically reduced 10 min after exposure of skin to the culture of S. epi plus glycerol. This result suggests that electron generated by glycerol fermentation of S. epi in skin may be able to eliminate the free radicals induced by UV. Our studies here provide the novel of biological roles of endogenous glycerol and skin beneficial bacteria in the UV-induced skin damages.
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