Effect of probiotic (Leuconostoc mesenteroides) bacteria on the regulation of blood glucose level in diabetic mice

• Main Authors: Anir Batsukh, 安貝蘇
• Other Authors: Eric Chun-Ming Huang
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/fpp48n

碩士 === 國立中央大學 === 生醫科學與工程學系 === 107 === Effect of probiotic (Leuconostoc mesenteroides) bacteria on the regulation of blood glucose level in diabetic mice Abstract: The aim of this study is to exam if probiotic bacteria can regulate the level of blood glucose in diabetic mice. Ten different bacterial strains were isolated from Aaruul, a Mongolian dried curd, and identified by 16S rRNA sequencing. These identified bacteria are Staphylococcus aureus, Bacillus sp, Bacillus simplex, Leuconostoc mesenteroides, Paenibacillus glucanolyticus, Staphylococcus hominis, Megasphaera massiliensis, Bacillus pumilus, Staphylococcus epidermidis, Enterobacter Xiangfangensis and Leuconostoc mesenteroides. Leuconostoc mesenteroides can survive in the room temperature without a freezer and at pH values in the range from 3 to 7. Results from in vitro experiments indicated that the bacteria can use glucose as a carbon source to trigger fermentation. It has been documented that bacterial fermentation can produce short-chain fatty acids (SCFAs) including butyric acid. Injection of mice with streptozotocin (STZ) dramatically increased the glucose in the blood, mimicking a phenotype of Type 1 diabetes. Administration of butyric acid by injection in abdominal ICR mice for a week increased insulin and decreased glucose in STZ-induced diabetic mice. In addition, administration of butyric acid lowered the level of IL-6, a cytokine which can promote islet β-cell dysfunction and abnormal glucose metabolism. Our study demonstrates for the first time that Leuconostoc mesenteroides is a probiotic bacterium and butyric acid acts as a beneficial probiotic metabolite for the treatment of hyperglycemia. Keywords: butyric acid, diabetes, Leuconostoc mesenteroides, probiotics, short chain fatty acids (SCFAs)