Effects of culture temperature on β-D-glucan and ergosterol production at room and high temperatures using Agaricus blazei Murill

• Main Authors: Yun-Yi Chen, 陳昀怡
• Other Authors: 徐敬衡
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/y77g3q

博士 === 國立中央大學 === 化學工程與材料工程學系 === 107 === In this study, the influence of temperature on the biomass concentration, products, especially bioactive substance, formation by using Agaricus blazei Murill was observed. According to the studies, β-(1,3)-D-glucan, one kind of polysaccharides, was famous for its effect of preventing cancer, diabetes, and hyperlipidaemia. The development of strategies in order to enhance the concentration of β-(1,3)-D-glucan was also conducted. We also discuss further about the influence of temperature on the concentration of ergosterol, other product of Agaricus blazei Murill, and we would like to know at which temperature ergosterol could achieve its highest productivity. Two strains of Agaricus blazei Murill, TITC1 and OKI1 were tested while the fermentation was conducted. The results showed that the best cultural condition to obtain high biomass concentration for TITC1 and OKI was at 25℃ and 30℃, respectively. The highest production of polysaccharide was acquired at 25℃ for TITC1 and at 35℃ for OKI1. To attain high production of β-(1,3)-D-glucan, OKI1 needed a higher temperature than TITC1. The highest production of β-(1,3)-D-glucan was acquired at 30℃ and at 35℃ for TITC1 and OKI1, respectively. Furthermore, we could get highest concentration of ergosterol at 25℃ and at 30℃ for TITC1 and OKI1, respectively.