Bio-inspired Porous Membrane via Self-Assembly and 3D Printing for Biomedical Applications

• Main Authors: Wang, Cheng-Heng, 王政衡
• Other Authors: Li, Ming-Chia
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/9nxg7m

碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 107 === In nature, there are many porous structures from animals to plants such as honeycomb, coral, bone, diatom shells. In these nature porous structure materials are providing substance exchange or reducing weight, they usually combine order and disorder in the hierarchical structure with stiff and soft materials, these characteristic dimensions spanning from the nanoscale to the macroscale. Taking advantage of the chemical and physical method, the bio-inspired porous structures can be obtained by top-down manufacture or bottom-up self-assembly. In this study, a bio-inspired porous 3D scaffold with different pore sizes spanning from the submicron to micrometers can be obtained by the combination of top-down 3D printing and bottom-up self-assembly for the biomedical application. To achieve the 3D scaffold with submicron pores, biocompatible and thermoplastic polyvinyl alcohol (PVA) polymer was used as a printing filament to additively manufacture a 3D-printed graft. Subsequently, water-insoluble and hygroscopic 3D-printed graft can be obtained via the PVA cross-linking by a biocompatible sodium trimetaphosphate (STMP). By taking advantage of sublimation, the biocompatible porous 3D scaffold structure can be obtained by the freeze-drying method. Combined 3D printing and freeze-drying, the 3D scaffold with 3D porous structure have been produced for mimicking the blood vessel with better substance exchange conditions. Furthermore, the chemical cross-linking polymeric hydrogel was imprinting on diacetylene-labeled polystyrene with micron porous structure which by taking advantage of self-assembly, a well-ordered, hexagonally packing and micron-sized pores can be achieved via the breath-figured method. Through a combination of top-down imprinting lithography, bottom-up breath-figured method, and freeze-drying the bio-inspired 3D porous structure in 2D membrane with the double-length-scale porous structures can be achieved. In these two ways, we can produce the two kinds of double-length-scale porous materials, one is in 3D scaffold the other is in the 2D membrane.