| Summary: | 碩士 === 國立暨南國際大學 === 應用化學系 === 107 === Polycyclic aromatic hydrocarbons originate from incomplete combustion or high temperature pyrolysis of fossil fuels and other carbonaceous fuels (such as wood and charcoal). They have strong chemical stability, resistance to natural degradation, bioaccumulation, and carcinogenic properties. The aromatic hydrocarbons can enter the surface runoff by municipal sewage, industrial wastewater, oil spill or leakage. Therefore, the determination of the types and concentrations of PAH in the environmental aqueous samples is of great importance for environmental pollution analysis.
MALDI-TOF MS is a powerful tool for the analysis of large biomolecules, but not for the analysis of small environmental pollutants such as PAHs. Here, polydopamine-coated nanodiamonds (PDA@ND) were synthesized and applied as both matrix and adsorbent for the analysis of PAHs by MALDI-TOF MS. The use of the PDA@ND as matrix can effectively improve the signal response of PAH including Benzo[a]pyrene (BaP), Benzo[g]perylene (BgP), and Pyrene and exhibit no background interference. A good linearity (R2 > 0.99) was obtained for these three PAHs and the limit of detection for BaP, BgP, and pyrene were 0.2, 0.02, 27.3 pg/μL, respectively.
Several important parameters for PDA@ND as adsorbent including amount of adsorbent, adsorption time, initial sample volume, and type of the eluent solvent were evaluated. The optimum extraction conditions were obtained as: 5 min for extraction time, 22 ng/10 g (BgP / PDA@ND) for loading capacity, 100 L for initial sample volume, and ACN as desorption solvent. We further validated the practicality by the analysis of PAHs in environmental water samples including tap water, farm ditch water, lake water, and industrial wastewater. The presented method by using PDA@ND as both matrix and adsorbent for MALDI-TOF MS analysis of PAHs proved to be a good candidate for simple and rapid determination of PAHs in environmental water, and served as an effective alternative to the existing GC-MS method for the determination of PAHs.
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