| Summary: | 碩士 === 國立成功大學 === 細胞生物與解剖學研究所 === 107 === Tendon is a type of connective tissue which is mainly composed of parallel collagen bundles through the direction of mechanical load. Tendon injury involves a slow repair process, which results in the poor intrinsic healing capacity. Surgical treatment of tendon ruptures and lacerations is currently the most common therapeutic treatment. Nowadays, researcher devoted to studying the restoration of tendon rupture by cell therapy, which has been suggested to be an ideal strategy. Scleraxis (Scx), a basic helix-loop-helix transcription factor, is expressed in the tendon/ligament cell population and positively regulates the expression of type I collagen (Col1) and tenomodulin (Tnmd) in tenocytes. Previous study has observed soluble factors such as connective tissue growth factor (CTGF) and bone morphogenetic protein 12 (BMP12) are demonstrated to be participated in the multiple stages of tendon healing process enhancing tendon repair. On the other hand, physical cue, such as mechanical force, is shown to enhance the differentiation ability for tendon tissue engineering. Although several studies have indicated, little attention has been paid whether the combination of physical force with growth factors has beneficial effect on tendon regeneration. Thus, the objective of this study was to investigate whether the combination of soluble factors with physical factor accelerates tenogenesis. We compared BMP12 with CTGF in different times and dosages. Examine protein expression level of tenogeneic related marker Scx, Tnmd and osteogenic marker Runx2. Result showed CTGF possess a positive impact and specificity on MSC tenogenesis. Micropatterning control cell shape with the aspect ratio 1:1, 3:1, 7:1. Immunofluorescence detected Scx expression in nuclear, Tnmd protein expression and statistic nuclear aspect ratio. Result showed the aspect ratio 7:1 could be the proper condition for MSC tenogenesis. In addition, shape control combines with CTGF advancing Scx located in nuclear. After inhibited F-actin polymerization by Cytochalasin D, indirectivity destructs the shape control tenogenesis, the expression of Scx in nuclear also been inhibited. in the future we will make sure the mechanism of cell shape combines CTGF accelerate tenogenesis. The present study may help understand the precise condition for tenogenesis and further provide a therapeutic strategy for tendon injury.
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