| Summary: | 碩士 === 國立中興大學 === 生醫工程研究所 === 107 === Basically, biosensing application of the surface plasmon resonance, there are two spectra results are achieved. For example, attaching a dye molecule directly to a metal nanoparticle surface typically results in fluorescence quenching due to energy transfer between the fluorophore and the metal. Thus the Raman spectrum of the molecule can be strongly enhanced due to the high electromagnetic field at the surface of the particle, namely surface enhance Raman Scattering (SERS). Alternatively, spacing the fluorophore slightly away from the particle surface prevents fluorescence quenching but can lead to a significant increase in the emission from the molecule, namely metal enhance fluorescence (MEF). That is, fluorescence emission for MEF and Raman scattering for LSPR are competing phenomena. In this thesis, I try to build up a platform, 3D nanostructure constructing from silver nanowire, for MEF and SERS detecting use. Depending on the arrangement between the nanostructures and the molecules, either the fluorescence or the Raman signal may be enhanced. First of all, I synthesis the nanowire and construct a campfire-bone liked 3D nanostructure, which allowed the analyst to be dropped or smeared on, and then the optimizes MEF or SERS effect was explored basing on the ratio between nanowire and analyst to find the detection limit, respectively. And then, I discuss and evaluate the MEF or SERS effect through molecules with variable positive-charge number and length of spacers base on the optimizes MEF or SERS platform. DNA binding molecules BMVC derivatives, pH biosensor BAP and standard lasing dye R6G were used as probes to develop the mechanism above. Meanwhile, both of the lasing wavelength of system and illumination degree of analyst (especial fluorescent organic nanoparticles, FONs) are also impact factors to collect appropriate data, which is analyst dependent. Eventually, the 3D nano-platform was successfully applied to detect the pesticides and bacteria, it is worth looking forward to evaluate the future application and development of this platform.
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