Detection of tau protein in the plasma of Alzheimer\'\'s disease patients by using magneto-optical immunoassay technique

• Main Authors: You-Jun Lin, 林宥均
• Other Authors: Kuen-Lin Chen
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5759007%22.&searchmode=basic

碩士 === 國立中興大學 === 奈米科學研究所 === 107 === Abstract Recently, γ-Fe2O3/Au core/shell (γ-Fe2O3@Au) nanoparticle has been widely used because of its special magneto-optical properties. Therefore, we utilized the magneto-optical Faraday effect of γ-Fe2O3@Au nanoparticle to detect the tau protein in the plasma of Alzheimer''s disease (AD) patient.We synthesized iron oxide (γ-Fe2O3) by co-precipitation method and deposited gold layer on the surface of γ-Fe2O3 magnetic nanoparticles by an iteration method to form gold-shell magnetic nanoparticles (γ-Fe2O3@Au MNPs). The tau antibody is modified on the surface of γ-Fe2O3@Au MNPs as a reagent for AD detection. Magneto-optical immunoassay technique is based on the Faraday rotation change induced by the clustering of MNPs. The clustering of MNPs is achieved via the antigen-antibody interaction of biomarker and biotarget [1].We found that the γ-Fe2O3@Au MNPs will enhance the magneto-optical Faraday effect [2], and the γ-Fe2O3@Au MNPs modified with the tau antibody can successfully detect the tau protein in the plasma of AD patient. We could successfully detect tau protein of clinical specimen and distinguish course of AD . Keyword:Alzheimer''s disease, biomarker, Faraday effect, tau protein. 1Kuen-Lin Chen, Yan-Shin Lin, Jian-Ming Chen, Chiu-Hsien Wu, Chien-Chung Jeng, Li-Min Wang. A sensitive platform for in vitro immunoassay based on biofunctionalized magnetic nanoparticles and magneto-optical Faraday effect. Sensors and Actuators B. 2018, 258, 947–951. 2Prashant K. Jain, Yanhong Xiao, Ronald Walsworth, Adam E. Cohen. Surface plasmon resonance enhanced magneto-optics (SuPREMO): Faraday rotation enhancement in gold-coated iron oxide nanocrystals. Nano Lett., 2009, 9, 1644-1650.