| Summary: | 碩士 === 國立中興大學 === 生物科技學研究所 === 107 === Pu-erh tea, famous for its special aroma produced by particular processes. Pu-erh tea has been shown to ameliorate hyperglycemia by inhibit -glucosidase pathway, thus considered a healthy drink. In addition, hyperglycemia is major symptom in diabetes mellitus, and stabilized postprandial blood glucose (PBG) is one of strategies to cure and prevent diabetes. Clinical drug, acarbose, has the gastrointestinal adverse effects, such as flatulence, diarrhea, abdominal discomfort, impair patient compliance and limit clinical utility. Thus, finding a less side effects drug is highly anticipated. In experiment, Pu-erh tea infusions were determined to inhibit -glucosidase activity, and raw Pu-erh tea inhibited about 90% activity in 25 g/mL. Though the HPLC analysis, raw Pu-erh tea is rich in strictinin, ((-)-epigallocatechin gallate, EGCG). In vitro, the IC50 values of strictinin and EGCG against -glucosidase were 2.4 g/mL and 4.3 g/mL. In addition, we discover that the inhibition type of strictinin on -glucosidase is mixed type. In vivo, strictinin improve oral sucrose tolerance in C57BL/6J mice at dose 100 mg/kg, but didn’t improve oral glucose tolerance. The results indicated that strictinin decreased the PBG level by though inhibit -glucosidase path-way. Therefore, strictinin may have the potential to prevent diabetes.
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