| Summary: | 碩士 === 國立中興大學 === 生物化學研究所 === 107 === Non-small cell lung cancer （NSCLC）is one of the most prevalent human mal- ignancies worldwide, with a high morbidity and mortality. Thymol has been rep- orted to exhibit antioxidant, anti-inflammatory, and anticancer activities. The pur- pose of this study was to investigate the anti-cancer effect of thymol on non-sm- all cell lung cancer. We used Thymol to treat lung cancer cells(A549, H1299) and to test the cytotoxicity with MTT assay. Among the two cell lines, human non-small cell lung cancer H1299 cells showed the most susceptibility to thymol. We also demonstrated that Thymol could induce the apoptosis of H1299 cell line by Propidine iodine and Annexin-V double staining experiments. In further examination with Western Blotting, Thymol could regulate the apoptosis by decreasing the protein levels of Bcl-2, Bcl-XL, and stimultaneously activate caspase-3, 9, PARP promoting apoptosis. The epithelial-mesenchymal transition(EMT) confers tumor cells with a distinct advantage for metastatic dissemination. Thymol downregulated N-cadherin, vimentin, Snail protein expressions. Moreover, Thymol inhibited the migration and invasion of H1299 cell. We confirm that Thymol do exhibit anticancer activity, expecting to provide a new drug design for cancer therapy.
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