| Summary: | 碩士 === 國立中興大學 === 生命科學系所 === 107 === Banana is a major food crop worldwide. However, Fusarium wilt is a serious pathogen and diminish banana production, including Pei-Chiao (AAA) which is an important commercial variety in Taiwan. Therefore, it’s an important task to protect banana from Fusarium oxysperum f.sp cubense infection. There is evidence that Brazil Xiangiao (AAA) induced systemic acquired resistance (SAR) by primary infection of Fusarium oxysperum f.sp cubense race1(Foc1) can resist Fusarium oxysperum f.sp cubense tropic race4 (Foc TR4) infection. In addition, we found that Pei-Chiao inoculated with Burkholderia cenocepaice sp. 869T2 not only promoted growth but also enhanced induced systemic resistance (ISR) against Foc4 infection. According to these studies, we aim to characterize the phenotype and transcriptomic of Pei-Chiao adaptive immunity induced by Foc1 and 869T2 to against Foc TR4 infection.
The in vitro test and field trial showed that the disease severity rating of Foc1 and 869T2 are both 30% lower than control. In transcriptomic analysis, the primary infection of Foc TR4 show the higher expression of defensed gene PR1 , downstream of SAR, compared to control. The 869T2 inoculation showed that PR2 and PR3 were upregulated, and the antibacterial substance HMB biosynthesis pathway and IAA and BR signal pathway were also activated based on enrichment anaylsis. Besides, the finding showed that the genes related to SA and JA-/ET- pathway were upregulated in both Foc1 and 869T2inoculation, which suggest that Pei-Chiao can resist the Foc TR4 invasion via SA and JA-/ET- pathway.
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