A study of pyrroloquinoline quinone on Pei-Chiao (Musa spp.) growth and biocontrol improvement

• Main Authors: Man-Yun Yu, 余曼筠
• Other Authors: 黃介辰
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/x3z4a6

碩士 === 國立中興大學 === 生命科學系所 === 107 === Banana is one of the most important food crops in the world, but it is deeply affected by the fusarial wilt, which leads to limited production. Therefore, solving this problem is the current priority. The results of previous experiments confirmed that the average plant height of Pei-Chiao with Burkholderia sp. 869T2 inoculated increased by 32.6 cm, and the incidence decreased by 21.1%. In this study, the effect of 869T2 inoculation on Pei-Chiao to promote growth and disease resistance was also verified in the greenhouse experiment, which effectively reduced the degree of fusarial wilt. In the transcriptome analysis, it was found that the genome of Pyroloquinoline quinone (PQQ) synthesized by 869T2 in vivo has high expression. The growth test of the Pei-Chiao was to explore whether PQQ is an important factor for promoting the growth of the Pei-Chiao and the role of resistan fusarial wilt. In promoting growth analysis, adding 100nM PQQ was proved that the plant height increased by 2.2 cm, the number of healthy leaves increased by 2.9 leaves, and the fresh weight increased by 1.1 g. In the greenhouse infection experiment, the average fusarial wilt in the PQQ was lower than that in the control. After adding PQQ to the transcript gene, the Pei-Chiao promoted the growth and biological control reaction. It was found that the addition of PQQ can improve carbohydrate metabolism, amino acid metabolism, photosynthesis, oxidation and high performance of gemcitabine synthetase. It may be a factor that promotes the growth of the northern banana and induces the performance of the plant resistance factor PR1.