Microbial community impact on carbon dioxide and methane flux at high salinity–A Case Study of the Beimen Salt Fields

• Main Authors: Xue-Yan Huang, 黃學彥
• Other Authors: Ben-Jei Tsuang
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5087054%22.&searchmode=basic

碩士 === 國立中興大學 === 環境工程學系所 === 107 === The wetland environment is an important carbon budget for natural environment. Methanogen plays an important role on methanogenesis. Although many studies have described the interaction between methanogens, Sulfate-Reducing Bacteria and Methane-Oxidizing Bacteria. But the relationship among microbial flora and methane and carbon dioxide flux in the high salinity environment is uncertainty; Therefor, we used previous Eddy Covariance system (EC) to observe the methane and carbon dioxide flux data of the salt field in the Beimen. Furthermore, the microbial sampling comparison and environmental factors are used to explore the correlation between methane and carbon dioxide fluxes in the salt field wetland and microbial communities. For water quality soil sampling analysis, we selected five place in the small evaporation pond of the experimental site to establish the environmental factors. The results show that the topsoil showed reddish-brown divalent iron oxide deposits and salt crystals. The deep black sludge with strong reduction at 2-7 cm below the topsoil proved that the microbial activity was significant. In the carbon sink function, the sampling period in the Low season is close to the seven lagoons and intertidal zones, and the sampling days in the Peak season are close to the fish farm. The daily carbon sequestration can be fixed at 0.14 and 0.08 g respectively during those operation periods. Finally, Next Generation Sequencing (NGS) is used to analyze the bacterial phase of each sampling point in Beimen salt field. Moreover, it can estimate relationship among microbial flora, methane and carbon dioxide flux in the environment.