Magnetic Graphitized Carbon Black Combined with Gas Chromatography-Tandem Mass Spectrometry for Determination of Hydroxyl Polycyclic Aromatic Hydrocarbons in Urine

• Main Authors: Pin-An Chen, 陳品安
• Other Authors: Maw-Rong Lee
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/g2t2x4

碩士 === 國立中興大學 === 化學系所 === 107 === In this study, a simple and sensitive analytical method using magnetic solid phase extraction (MSPE) combined with gas chromatography–tandem mass spectrometry (GC-MS/MS) was developed to determine hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs) in human urine. The magnetic graphitized carbon black (MGCB) was utilized as sorbent in dispersive solid phase extraction to extract OH-PAHs in urine sample. The results showed that OH-PAHs in 2 mL urine sample was extracted by 10 mg/mL MGCB under sonicated for 10 seconds. Subsequently, the adsorbed OH-PAHs were eluted by using 500 µL ethyl acetate twice under sonicated for 20 second. After derivatization step, the analytes were ionized by electron ionization (EI) and quantified by using selected reaction monitoring (SRM). Under the optimal extraction and detection conditions, the linear range of the proposed method for detection of OH-PAHs were between 1-20000 pg/mL with coefficient of determination above 0.9933. The intra-day and inter-day precisions were 0.3-7.7% and 1.9-18.2%, respectively. The limit of detection (LOD) were in the range of 0.2-33 pg/mL. The proposed method was tested by analyzing of trace OH-PAHs in real human urine samples. The trace OH-PAHs were successfully detected by the proposed method with concentration between 1-59715 pg/mL. The results showed the proposed method can be applied to determine the trace OH-PAHs in human urine.