Overexpression of Enterocin SE-K4 in Arabidopsis Enhance Chilling Tolerance and Growth Inhibition of Listeria Monocytogenes

• Main Authors: YEN, HSI-CHENG, 閻璽丞
• Other Authors: CHIANG, CHIH-MING
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/t89j6a

碩士 === 銘傳大學 === 生物科技學系碩士班 === 107 === Bacteriocins can inhibit the growth of harmful bacteria in foods, such as Listeria monocytogenes. Bacteriocins produced by yeasts in pickles that can inhibit the growth of other bacteria. Enterocin SE-K4 is from Enterococcus faecalis K-4, which is known to have heat tolerance and inhibition of Enterococcus faecium, Enterococcus faecalis, Bacillus subtilis, Clostridium beijerinckii, and Listeria monocytogenes. We transferred Enterocin SE-K4 gene to Arabidopsis thaliana by Agrobacterium-mediated transformation . Genomic PCR has been used to prove seven transgenic plants were identified. Real time -qPCR was used to confirm that Enterocin SE-K4 expressed in Arabidopsis thaliana. Overexpression of Enterocin SE-K4 increase chilling tolerance of Arabidopsis thaliana and crude extracts of Enterocin SE-K4 transgenic plants inhibit Listeria monocytogenes.