| Summary: | 碩士 === 銘傳大學 === 生物科技學系碩士班 === 107 === Bacteriocins can inhibit the growth of harmful bacteria in foods, such as Listeria
monocytogenes. Bacteriocins produced by yeasts in pickles that can inhibit the growth
of other bacteria. Enterocin SE-K4 is from Enterococcus faecalis K-4, which is known
to have heat tolerance and inhibition of Enterococcus faecium, Enterococcus faecalis,
Bacillus subtilis, Clostridium beijerinckii, and Listeria monocytogenes. We transferred
Enterocin SE-K4 gene to Arabidopsis thaliana by Agrobacterium-mediated
transformation . Genomic PCR has been used to prove seven transgenic plants were
identified. Real time -qPCR was used to confirm that Enterocin SE-K4 expressed in
Arabidopsis thaliana.
Overexpression of Enterocin SE-K4 increase chilling tolerance of Arabidopsis thaliana
and crude extracts of Enterocin SE-K4 transgenic plants inhibit Listeria monocytogenes.
|
|---|
