| Summary: | 碩士 === 義守大學 === 生物科技學系 === 107 === Tomato is an important fruit crop in Taiwan. Taiwan is located in the subtropical region. The high temperature and high humidity should be beneficial to the growth of plant pathogens, including Xanthomonas spp. The bacterial spot has caused serious tomato diseases in the world. In the past, the way to prevent and treat this disease was mainly using copper-containing agents. But e long-term use of copper-containing agents has already caused many bacterial spot pathogens to develop resistance. In addition, the speed of transmission is always fast, and the speed of prevention cannot stop the speed of disease diffusion. Excessive use of pesticides is also likely to cause environmental hazards and possibly harm human. Several bacterial isolates were collected and isolated from leaf lesions of chili and tomato plants from commercial fields in Kaoshiung and Pintung areas. These isolates were ferther confirmed their pathogenicity through Koch’s postulated on its host plants under controlled environment and the isolates were subsequently identified as Xanthomonas euvesicatoria (XE) and X. perforans (XP) using polymerase chain reaction. In seeking microorganism that has biocontrol property to fight with Xanthomonas euvesicatoria (XE) and X. perforans. They are Bacillus atrophaeus(01)、Bacillus mojavensis(08)、Burkholderia vietnamiensis(44)、Bacillus atrophaeus(46) . In vitro, they all expressed strong inhibition property against leaf spot bacteria(p < 0.05). However, the preliminary test in greenhouse showed that these four bacteria only have protective effect but no curative property.
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