| Summary: | 博士 === 義守大學 === 化學工程學系暨生物技術與化學工程研究所 === 107 === Separation and purification is the basic technology for industry. It works for the pretreatment of raw materials and the purification of final products for petrochemicals, specialities, biotechnologies, pharmaceuticals. Compared to distillation and membrane separation, chromatography is more widely apply in biotechnology and pharmaceutical industries because of low-temperature operation and high selectivity of adsorbent. SMB(Simulated Moving Bed), one of the continuous chromatographic technique, can greatly increase the productivity of solid adsorbent and reduce the solvent consumption. SMB gains its importance in the last two decade in pharmaceutical industry.
This study is divided into two major parts. The first part aims to combine the use of supercritical fluid and the simulated moving bed, and the second part aims to use a 6 zones SMB with an additional CIP section for the ternary separation with very strong retention components in the feedstock.
SF-SMB is a continuous chromatography with supercritical fluid as the desorbent, which can avoid the problem of high energy consumption for the down stream concentration and solvent recovery. By using the SF-SMB, which was designed and manufactured in our laboratory, sesamin and sesamolin can be separated, and the Triterpenoids can also be separated from the crude extract of Taiwanofugus camphorata. The results show that the purity of sesamin is 99.4% and the recovery is 98.1%. In the study of separating Triterpenoids from other impurities, the results reveal that the design of grad-SF-SMB with silica has the highest effectiveness on removing the impurities. It is also found that an iso-SF-SMB with silica also can successfully remove the impurities, and an iso-SF-SMB with RP-18 can also be used for removing the impurities with little lost of the triterpenoids. However, a traditional SMB with RP-18 is not suitable for removing the impurities. Both studies demonstrate that SF-SMB would be a useful technology for developing botanical drugs and provides greener alternative for industries.
The second part of this thesis aims to use a SMB cascade for the multi-component separation. This study presents two three-step procedures for the SMB cascade to separate the intermediate retention component from a feedstock with very strong retention component. The feedstock is classified into two categories: (A) KA＜KB＜＜KC; and (B) KA＜＜KB＜KC. Based on the procedures, this study can successfully separate the intermediate retention component from two crude extracts. The experimental results for both crude extracts shows that the separation of the intermediate retention component with near 90% purity and recovery can be achieved without knowing the adsorption isotherms foe each impurity. This study provides a quick and simple method to operate a SMB cascade for the ternary separation.
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