The study of tyrosinase production by recombinant E. coli
碩士 === 輔英科技大學 === 生物科技系碩士班 === 107 === The tyrosinase has a molecular weight of 75 kDa and is a polyphenol oxidase (PPO) containing copper ions. The active center is coordinated with two copper ions (CuA and CuB) to support six histidines. For example: synthetic dopamine, environmental treatment or...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2019
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| Online Access: | http://ndltd.ncl.edu.tw/handle/932kqs |
| Summary: | 碩士 === 輔英科技大學 === 生物科技系碩士班 === 107 === The tyrosinase has a molecular weight of 75 kDa and is a polyphenol oxidase (PPO) containing copper ions. The active center is coordinated with two copper ions (CuA and CuB) to support six histidines. For example: synthetic dopamine, environmental treatment or phenolic compound detection. This study used recombinant E. coli to produce B. megaterium tyrosinase to produce tyrosinase. The effect of tyrosinase activity on different growth time and transformation temperature. And use Bacterial cellulose immobilized cells and tyrosinase were evaluated as possibilities for biosensors.
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