Effects of Iron Chelator Deferoxamine on Metabolic Reprogramming and Lipid Droplets Formation in Human Breast Cancer Cells MCF-7

• Main Authors: YEH, CHIA-FENG, 葉嘉豐
• Other Authors: LIEW, YIH-FONG
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/m98bn7

碩士 === 輔仁大學 === 營養科學系碩士班 === 107 === Cancer cells require more iron than normal cells due to their rapidly growth, it leads to iron chelating potentially applying in cancer therapy. Metabolic reprogramming is an important characteristic for cancer cells, but also a key affecting factors for cell fate decisions. Because the altered metabolic response is associated with the regulation of protein expression. Therefore, we employed proteomics-metabolomics combined approach to explore the link between iron chelating-mediated cell growth and cell metabolism in human breast cancer cells MCF-7. Integrated both of metabolome and stable isotope dimethyl labeling proteomics coupled with LC-MS/MS results indicated that glycolysis, tricarboxylic acid cycle, electron transport chain and glutaminolysis are significantly altered in MCF-7 cells after DFO treatment for 48h. On the other hand, the intracellular lipid droplet accumulation was observed in DFO-treated cells. Lipid desaturase SCD1 was increased after DFO treatment. In contrast, metabolic regulator AMPK and p-AMPK were reduced in DFO-treated cells. In addition, mitochondrial inner membrane fusion protein OPA1 was also reduced in DFO-treated cells. In summary, iron chelator treatment results in mitochondrial dysfunction and metabolic reprogramming, leading to lipid droplet formation and affect cell growth in breast cancer cells.