| Summary: | 博士 === 大葉大學 === 生物科技與產業博士學位學程 === 107 === Production and efficacy of bioactive compounds form Cordyceps militaris (C. militaris) fruiting bodies and its fermentation grain was usually variants due to different strains. Through comparison of the anti-proliferative, apoptotic, and antioxidative properties of ethanolic extracts of fruiting bodies (FBE) and its fermented rice (FRE) from two strains of C. militaris applied to human breast cancer cell lines (MCF-7 or MDA-MB-231).
We revealed that FRE of the Zhangzhou strain (FRE-Z) produced a high level of cordycepin and exhibited comprehensive in vitro antioxidant activity against DPPH, superoxide radicals, hydroxyls, and low-density lipoprotein oxidation. Only FRE-Z showed dose-dependent inhibition of cell proliferation, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyl tetrazolium bromide assay in MCF-7 (0.7 mg/mL) and MDA-MB-231 cells (1 mg/mL) after 24-h culture. The anti-proliferative effect of FRE-Z were associated with an early stage of apoptosis induction at 4 h of treatment with 0.5 mg/mL FRE-Z in MCF-7 cells, as determined using annexin V and propidium iodide staining of apoptotic cells. The anti-proliferative effect was determined to occur through p53 activation but not through the release of mitochondrial apoptosis-inducing factor or caspase-9 activation for an initial culture period of 16 h. In addition to transient increase in cellular antioxidant enzyme, Cu/Zn superoxide dismutase was found in MCF-7 cells after 2-h treatment with FRE-Z. Therefore, FRE-Z exhibited various dose-dependent and exposure-time-dependent activities and has potential for application in breast cancer chemoprevention.
On the other part of study related to ACEI activity in edible mushroom, four edible or medicinal mushrooms: Flammulina velutipes, Pleurotus ostreatus, Pleurotus eryngii, Cordyceps militaris/ fermented brown rice base were treated in difference condition and extraction medium. We found the best ACEI activity in F. velutipes in powder form and under ethanol extraction for 1 h was 86.9 %. In 6 h extraction time, the higher ACEI activity was noticed in P. ostreatus in powder form and ethanol extraction was 76.1 %.
On our conclusion, strain of C. militaris had had a high concentration of cordycepin in the FRE-Z (14.36 mg/g) and had a higher anti-oxidation effect. In anti-cancer effect, FRE-Z was showed a higher cytotoxic activity in both noninvasive MCF-7 and aggressive MDA-MB-231 breast cancer cell lines. The mechanism was related to induce expression in FRE-Z of the enzymatic antioxidants copper and zinc superoxide dismutase 1 (SOD1) and activation of the p53-mediated apoptosis pathway. Strain of C. militaris Zhangzhou with high-producing medium of fermented rice had served as an efficient source of cordycepin for breast cancer therapy.
On the other hand, increasing reaction surface (powder from) and shorten extraction time (1 h) would get a better ACEI activity.
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