Sulfonated Polyaniline Scaffolds for Neural Differentiation of PC12 Cells under Electrical Stimulation

• Main Authors: Rui-Da Chen, 陳睿達
• Other Authors: Jui-Ming Yeh
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/v7ha38

碩士 === 中原大學 === 奈米科技碩士學位學程 === 107 === Sulfonated polyaniline was synthesized by oxidative polymerization and PC12 cell was cultured on the film of sulfonated polyaniline in this experiment, this study would observe the influences on the differentiation of PC12 cell by controlling the voltages and amount of sulfonate group in polyaniline chain Proceeding the structure identification by Fourier Transform Infrared Spectrometer and Elemental analysis, measuring the properties with Contact Angle, Cyclic Voltammetry and Electric Conductivity, this study found out sulfonated polyaniline had reversible doping and reversible reduction-oxidation which was known as a characteristic of conductive polymer. For further research about the effects of neuron regeneration, PC12 cell was cultured on material of sulfonated polyaniline, the adhesion rate and cell viability increased as sulfonation rate had increased under the experiments of adhesion and cell viability. After calculating neurite length by microscope and immunofluorescence, the best differentiation effects came from the sulfonated polyaniline cotted with laminin.