| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 107 === The applications of non-thermal dielectric barrier discharge plasma (DBD) have been used widely in biomedical area nowadays. Throughout the wound healing period, the wound should avoid to any bacterial infection during the inflammation stage and the fibroblast plays an important role in the proliferation phase. Therefore, the study is to develop a non-thermal atmospheric planar plasma system. The ameliorate of the nozzle on the planar reactor can produce plasma under atmospheric pressure and it has been proved to have high sensitivity to the cells with high sterilization effect. Using the optical emission spectroscopy, the singlet oxygen can be detected under 777, 845 and 927 nm wavelength and 297 and 309 nm for free radical species. In 600 s and 34ºC excitation of plasma, it will not cause any thermal damage to tissues or wound. While the migration rate of the fibroblast is found to be best under 300s of treatment throughout 36 h observation on the cultivation. Among them, 30s and 60s are the best parameters for the period of treatment, which increase of 51.2 and 50.2 µm cell movement distance respectively. The study also showed on the inactivation of E. coli and S. aureus can achieve D-value under 300s and 480s respectively and complete sterilization when the time is over 300s and 540s for E. coli and S. aureus respectively. In comprehensive of the result, it is suggested to treat the wound with plasma during inflammation phase for 300s, in order to achieve 99.99% of E. coli and 70.15% of S. aureus sterilization effect, but the activity of fibroblast will be decreased by 25%, migration rate increased by 19.32%. In 60s of treatment during proliferative phase, the activity of the fibroblast is increased by 50%, migration rate increased by 42.11%, but the sterilization effect on the E. coli and S. aureus can only achieve 44.58% and 28.07% respectively. It is recommended to use suggested parameters matching with inflammation phase and proliferative phase for the best effect on wound healing.
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