The Bioactive Evaluation of the Extract Flowers of Musa paradisiaca

• Main Authors: WANG, KO-HSUAN, 王可璇
• Other Authors: HUANG, HO-CHENG
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/e4cj5h

碩士 === 正修科技大學 === 化妝品與時尚彩妝研究所 === 107 === Banana (Musa paradisiaca), a perennial monocotyledonous herb, is popular as a highly nutritious plant in tropical and subtropical regions. However, banana flower robs its nutrients during the growth of bananas and is therefore removed and discarded as agricultural waste. Banana plants themselves contain biologically active compounds such as vitamins C, E, phenolic compounds, carotenoids, biogenic amines, plant sterols, minerals, and dietary fiber. In this study, the antioxidant components, ability, inhibition of tyrosinase and moisturizing ability of the extracted banana flower extracts were extracted by different solvents, and the antioxidant capacity of the extract obtained by the process of concentration, purification and separation of the ethanol extract of banana flower was investigated. After the test, the results of the extract were compared under the conditions of the test. The experimental results showed that the antioxidant capacity of the extract was determined by DPPH free radical scavenging ability, and the clearance rate was ethanol extract (94.57%) > ethyl acetate extract (69.56 %) > water extract removal power (61.64%); The clearance of banana flower extract after separation and purification was B3 extract (96.54%) > B1 extract (13.83 %) > B2 extract (9.62%). The chelating ability of ferrous ions was determined by ethanol extract (95.25 %) > ethyl acetate extract (78.05 %) > water extract (53.49%); after extraction and purification, the chelation ability of banana flower extract was extracted by B3 (96.61%) > B1 extract (89.76 %) > B2 extract (82.09%). Reducing power was determined by ethanol extract (0.811) > ethyl acetate extract (0.455) > water extract (0.267); reduction and purification of banana flower extract after separation and purification with B3 extract (0.907) > B1 Extract (0.196) > B2 extract (0.138). Antioxidant component of banana flower extract, total phenolic content as ethanol extract (89.6 mg GA/g d.w.) > water extract (16.8 mg GA/g d.w.) > ethyl acetate extract (8.32 mg GA) /g d.w.). The total flavonoid content was determined as ethanol extract (4.9 mg Rutin/g d.w.) > water extract (5.10 mg Rutin/g d.w.) > ethyl acetate extract (3.68 mg Rutin/g d.w.). The polysaccharide content test was carried out by measuring the total sugar content of the standard glucose, the total sugar content of the water extract (59.52 glucose/g d.w.); the determination of the reducing sugar content by the standard glucose, and the reducing sugar content of the water extract was (8.16 glucose/ g d.w.). Inhibition of the tyrosinase assay in vitro, inhibition rate of ethanol extract (79.79 %) > ethyl acetate extract (51.61%) > water extract (43.16%). The water absorption test was carried out with water extract (16.96 %) > ethyl acetate extract (1.44%) > ethanol extract (1.26%). The moisture retention test was carried out with water extract (45.52%) ethyl acetate extract (27.78%) > ethanol extract (26.98 %).