| Summary: | 碩士 === 嘉南藥理大學 === 保健營養系 === 107 === Epstein-Barr virus ( EBV ), the human herpesvirus type 4 ( HHV-4 ), is one of the most common viruses in humans. About 95% of adults worldwide are infected with infectious mononucleosis. Various tumor diseases such as nasopharyngeal carcinoma are highly correlated. Previously, it was found that the ethyl acetate extraction F3a of Polygonum cuspidatum increased the cleavaged caspase-3 and PARP by inhibiting NF-B, and the EB virus tumor cells were apoptotic. Analysis of the main component of F3a as emodin by reverse high-performance liquid chromatography ( HPLC ). Salvia miltiorrhiza is a plant of the family Labiatae, in which tanshinone is an anti-cancer, anti-bacterial, anti-viral and cardiovascular-protecting activity component. Previous studies in our laboratory have investigated the toxicity of tanshinone IIA to EB virus tumor cells at concentrations above 0.5 μg / mL. Both emodin and tanshinone IIA have anti-cancer and anti-viral effects, so this study will further explore whether emodin and tanshinone IIA have synergistic effects on EB virus tumor cells. Emodin and tanshinone IIA have a synergistic cytotoxic effect on Epstein-Barr virus tumor cells-Raji cells. The combination of 37 μM emodin and 0.85 or 1.7 μM tanshinone IIA synergistically inhibits tumor cell growth and sustained 48 hours. It can also reduce the gene expression of EBNA1 and BNLF1, the expression of p-IB / IB and p-ERK / ERK protein, and the apoptosis-related proteins PARP and caspase-3 cleavage are significantly increased, and the cells are that survival and proliferation decline and progress toward apoptosis.
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