| Summary: | 碩士 === 嘉南藥理大學 === 化粧品應用與管理系 === 107 === Dysfunction of the epidermal barrier of the skin, such as sebum deficiency or imbalance of keratinocyte proliferation and differentiation, can lead to skin diseases, for example, atopic dermatitis, psoriasis and porokeratosis. The extracts of Chinese herbal medicine have been reported to be alternative drugs. S. baicalensis extracts contain various flavonoids and have anti-inflammatory, antioxidant, anti-cancer, antibacterial, anti-viral, and anti-aging effects.
Oroxylin A (5,7-dihydroxy-6-methoxyflavone), the least abundant flavonoid in S. baicalensis extracts, has a wide range of pharmacological activities, including anti-inflammatory, anti-cancer, anti-allergic and antibacterial effects. Oroxylin A has the inhibitory activity of the transport of melanosome and reducing the intracellular melanin content, but lacks the relevant literature on the application of oroxylin A to the stratum corneum of the skin. Oroxylin A has a rare natural source and is very cumbersome to extract. Previous studies showed different synthetic methods to produce oroxylin A, but the synthetic conditions are highly toxic and explosive. In this study, a milder, time-consuming, safe and high-yield one-pot reaction was used to synthesize the required oroxylin A.
Keratinocytes were treated with different concentrations of oroxylin A, and there was no cytotoxicity in the periods of treatments. Human whole-genome microarray analysis was used to identify significantly expressed genes that may contribute to the response, and then the gene ontology enrichment analysis could perform the signaling pathways by gene expression profiling. After quantitative polymerase chain reaction analysis, the results indicated lipid metabolism and the regulation of differentiation and proliferation of keratinocytes maybe involved. Therefore, we speculated that when the skin is stimulated by environmental changes or external mechanical stress, if it can accelerate the metabolism of keratinocytes, it might be able to achieve the effect of repairing skin abnormalities. In addition, the gene microarray analysis used in this study can be used to clarify the possible regulatory pathways of the compounds administered to cells, which can be applied to evaluate the safety and effectiveness of raw materials of cosmetics for the skin in the future.
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