| Summary: | 碩士 === 長庚大學 === 顯微手術國際碩士學位學程 === 107 === Introduction: Mice have advantages over other animals, such as low costs,
easy handling, and adequate laboratory resources. However, mice
vascularized composite allotransplantation (VCA) surgery is not popular,
partly because of technical difficulties and high mortality rates. Our goal
was to demonstrate a face transplantation model in mice that includes both
vascularized mandibular bone and oral mucosa.
Methods: We proposed a new face allotransplantation model composed of
oral commissure, mandible, teeth and oral mucosa in C57BL/6 mice. The
length of the right incisor on the transplanted mandibles were measured,
CT scan was performed, and different tissue samples were obtained, for
histologic evaluation.
Results: We performed 5 consecutive transplantations. The mean operative
time for the donor was 80 min, and for the recipient was 123 min. There
were neither flap failures nor animal deaths. The follow up was 6 months.
The right incisor of the transplant grew at different rates in all cases.
Histology samples showed viability in all tissues, including mandibular
bone marrow. CT scan demonstrated preservation of the transplanted bone
structure.
Conclusion: Our study proposed a reliable mouse-model that can be further
used for immunologic research in face allotransplantation.
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