Drug delivery and pharmacokinetics study using curcumin loaded layer-by-layer nanoemulsions

• Main Authors: Hsin Ting Lin, 林鑫廷
• Other Authors: C. H. Liu
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05114097%22.&searchmode=basic

碩士 === 長庚大學 === 生物醫學工程研究所 === 107 === Curcumin has medical potentials for a variety of inflammatory diseases, but its therapeutic effect is low due to its low aqueous solubility and fast metabolism. In this study, curcumin was coated using layer-by-layer nanoemulsions to evaluate its bioavailability and stability. A variety of surfactants and polymers were screened for the layer-by-layer self-assembly nanoemulsions. The cationic surfactant dioctadecyldimethylammonium bromide (DDAB), sodium carboxymethyl cellulose (CMC) and chitosan (CHIT) was used in the formulations to evaluate the effects of different layers of coating. The concentration of DDAB/CMC/CHIT/CMC/CHIT of the layer-by-layer nanoemulsion were 0.1%, 0.06%, 0.06%, 0.09% and 0.09% (W/V), respectively. The particle size, zeta potential, cytotoxicity, emulsion delamination index, viscosity, conductive plating, coating efficiency, contact angle and surface tension were analyzed to understand the physicochemical properties of the layer-by-layer nanoemulsions. In the simulated intestinal fluid, the results slowed a slow release rate and a minimum release using a 2-layer nanoemulsion. Both the anti-oxidation test and the chelating analysis showed that when the anionic CMC was in the outermost layer, it had better antioxidant capacity, and the chelating ability. Curcumin can increase the antioxidant capacity through layer-by-layer coating. In the hemolysis experiment, the CMC coating can reduce the hemolysis rate as compared to the control nanoemulsion. Animal experiments of oral administration to rats show, layer-by-layer coating can improve the bioavailability of curcumin.