| Summary: | 碩士 === 長庚科技大學 === 健康產業科技研究所 === 107 === Alzheimer's disease (AD) is a progressive neurodegenerative disease, the most common dementia, and many studies support Aβ as a causative factor in AD. Aβ is considered to be a necessary pathogenic factor but not enough to fully explain the pathogenesis of the disease, so it is still to be further studied and clarified. Acrolein (Acr) is one of the lipid peroxides. It is widely found in environmental pollutants. It is cytotoxic to various organs or tissues of the human body. It is also a strong electrophilic compound forming a type of post-translational modification (PTM). Therefore, the primary objective of this study was to detect Aβ-Acr adducts and their associated autoantibodies in serum samples from AD patients and healthy control (HC) subjects. In this study, the serum of AD and HC groups were analyzed using ELISA. Our results showed no significant difference in serum total protein concentration between the two groups, which was in line with the experimental expectation. There was also no significant difference in total serum Acr-protein adducts between the two groups, suggesting that human serum normally contains acr-protein adducts. In the contrary, there was a significant increase in the Aβ-Acr adduct as well as IgG and/or IgM against Acr-modified adducts in the serum of AD patients. Thus, the increase of Acr-modified adducts and the IgG and IgM of anti-Acr-modified adducts in serum may be developed as early AD markers for diagnosis.
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