The anti-inflammatory effects of carnosic acid in human neutrophils

• Main Authors: HSU, YUN-HSUAN, 徐芸萱
• Other Authors: HWANG, TSONG-LONG
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/dex2hg

碩士 === 長庚科技大學 === 健康產業科技研究所 === 107 === Deregulation of immune system causes neutrophilic inflammation, resulting in various inflammatory diseases. Targeting neutrophilic inflammation is the latest strategy for the treatment of acute respiratory distress syndrome (ARDS) because pulmonary infiltration of neutrophils is a key feature of ARDS. Carnosic acid (CA), a phenolic diterpene, is abundant in plants of the Lamiaceae family. In previous studies, CA exhibited antioxidant and antibacterial properties. In our current investigation, we found that CA significantly inhibited superoxide anion generation, elastase release, CD11b expression, and adhesion in human neutrophils. CA also decreased the formation of neutrophil extracellular traps (NETs). Further experiments confirmed that CA inhibited neutrophilic inflammation by inhibiting the MAPKs signaling pathway. Moreover, CA effectively scavenged reactive oxygen species (ROS) and antagonized formyl peptide receptor 1. In ARDS animal model, CA improved lung tissue damage by significantly decreasing the infiltration and inflammatory response of neutrophils in the lungs. Together, these results demonstrate that CA exhibits anti-inflammatory potential via suppressing respiratory burst, degranulation, chemotaxis, and NET formation in human neutrophils. According to our finding, CA could be used to treat or attenuate the symptoms of ARDS, and thus could be developed as a dietary supplement or serve as a lead natural compound for the adjuvant treatment of pulmonary inflammatory diseases.