| Summary: | 碩士 === 國立中正大學 === 光機電整合工程研究所 === 107 === For early diagnosis of cancer, it is extremely important to establish stable and highly accurate results analytical methods for screening cancer biomarkers. Human cardiac troponin I (cTnI) is considered to be the gold standard for the diagnosis of acute myocardial infarction (AMI) because AMI is caused only by myocardial damage.
A common detection method is the enzyme-linked immunosorbent assay (ELISA), but this method still has some challenges in accurate diagnosis. For example, ELISA can only measure one target at a time, poor stability, and high limit of detection. Therefore, the development of array-based multi-point analysis can take signal from multiple biomarkers and improve the analytical performance.
Nd3+ doped up-conversion nanoparticle (UCNPs) has great potential for bio-sensing. Because the absorption wavelength of UCNPs is located at 793 nm, which has high tissue penetration depth and low tissue overheating effects.
In addition, the UCNPs is combined with the water environment detection platform of the low refractive index resonant waveguide grating (RWG), and the GMR effect causes strong local field on the surface of the waveguide layer, which will greatly increase the luminescence signal and reduce the limit of detection. The RCWA simulation is used to optimize the thickness of RWG to achieve the maximum of the electric field intensity .
In this study, UCNPs for cTnI sandwich Type immunoassay combine with low refractive index RWG based planar array biosensor is developed. The result show that the limit of detection is 0.0089pg, and the cTnI in the serum is detected. Medically, the amount of cTnI in normal human is less than 30pg, it mean the biosensor which reported in this study can be used for early diagnosis of AMI.
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