Preparation of Corn (Zea mays) Synthetic Seeds and Evaluation of Survival Rate after Low Temperature Preservation

• Main Authors: WANG, CHUN-SHENG, 王俊升
• Other Authors: CHANG, HSIANG
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/c34mvw

碩士 === 元培醫事科技大學 === 生物科技暨製藥技術系碩士班 === 106 === With the emerging technology, human life is getting convenient, however, environment damages are getting worse as well. There are many valued food crops today that have either been extinct or endanger due to environmental changes and the development of human civilization. In order to preserve important food species, the purpose of this project is to create artificial seeds from corn embryos coated with 3% Sodium Alginate and 25 mM Calcium chloride . The artificial seeds were stored in PVS2 (Plant Vitrification Solution 2) that was composed of 30% glycerol、15% DMSO (dimethyl sulfoxide)、15% ethylene glycol, and various amount of sucrose to final 0 M, 0.2 M, 0.4 M and 1.0 M of sucrose respectively. These artificial seeds stored in PVS2 were then subjected to low temperature treatment at -20 °C. After 3 weeks, the artificial seed survival rate was evaluated by tissue culture. Recovery rate was assessed through tissue culture technique to determine the protective effects of cryoprotectant against chilling injury on artificial corn seeds. Artificial corn seeds can be recovered successfully after 3 weeks under -20 ºC storage. The results of this study can provide information for future artificial corn seed production and serve as a reference for cryo-preservation.