| Summary: | 碩士 === 元培醫事科技大學 === 生物科技暨製藥技術系碩士班 === 106 === The protection of Sesamin derivative –BBD against UV-stress on ARPE-19 cells.
Student：Yi-Chen Wang Advisor：Chien-Wei Hou, Ph. D.
Department of Biotechnology and Pharmceutical Technology
Yuanpei University of Medical Technology
Abstract
In the present study, we used sesamin derivatives, 3-bis (3-methoxybenzyl)-butane-1,4-diol, (BBD) as the subject, and analysis its protect effect from injury from irradiation on retinal pigment epithelial cell (ARPE-19 Cells).The cell viability (MTT) and cytotoxicity (LDH) were measured after incubation with the BBD for 24 hours and then subjected to irradiation with ultraviolet light (100 MJ). Apoptosis of ARPE-19 Cells was induced by UV irradiation (100 MJ), and reactive oxygen species (ROS) were generated. The protein expression of MAPKs, Caspase-3, and Bax was analyzed by Western blotting. The results showed that the cell viability (MTT) of ARPE-19 Cells was significantly decreased after UV-stimulation, and the cell viability was significantly increased with dose-dependent of BBD concentration. Our study showed that BBD pre-treatment can protect RPE cells from UV-induced apoptosis. The present study suggests that the BBD in the prevention of retinal diseases associated with UV-stimulation induced oxidative stress such as AMD disease.
Keywords：Sesamin derivative –BBD、Apoptosis、RPE、UV
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