| Summary: | 碩士 === 國立雲林科技大學 === 化學工程與材料工程系 === 106 === Petroleum is currently the main source of energy and chemical for human society. However, the global warming and climate change resulted from petroleum exploitation are impairing the sustainability of future. Biorefinery which uses renewable biomass to replace petroleum as feedstock to produce chemicals and fuels is considered as solution for sustainable manufacturing. In order to achieve the goal of sustainable biomanufacturing, the research on microbial metabolic engineering for renewable chemical production is rapidly evolving.
Nevertheless, whole cell metabolism generally encounters a number of challenges, including low volumetric productivity, the formation of toxic intermediates or products. In vitro biosynthesis is not limited by cells and therefore has many attractive advantages including fast reaction rate, high yield, and easy control of reaction conditions. However, in vitro biosynthesis usually needs ATP addition or expensive phosphorylated donors.
In this study, an ATP independent pathway was designed to produce 3-Hydroxypropionic acid (3-HP) from maltodextrin through in vitro biosynthesis. Using PPi-PFK to replace ATP dependent phosphofructokinase in the synthetic pathway has two advantages: (1) utilizing pyrophosphate as a phosphate donor is cheaper than ATP, and (2) the phosphate released from by PPi-PFK and GPP1 can be reused by α-GP resulting nearly stable pH and phosphate concentration. Although the process needs to be improved and optimized, it is clearly that the expression of proteins and determination of enzyme activity has been done successfully.
|
|---|
