| Summary: | 碩士 === 國立陽明大學 === 生物醫學資訊研究所 === 106 === Wood formation efficiency can increase as plant drought resistance ability increases, so we tried to find out drought resistance related genes. Our laboratory conducted RNA-seq data of Populus trichocarpa drought treatment groups and their control group to obtain a set of differentially expressed genes (DEGs) including transcription factors (TFs). These drought response TFs were used to build a transcriptional regulatory network for wood formation.
In order to identify interactions between TFs and promoters to construct a regulatory network, our laboratory developed a novel yeast one-hybrid (Y1H) system, Meiosis-directed Y1H (hereinafter called Meiosis), which integrated the advantages of the two traditional Y1H systems, Haploid transformation (hereinafter called Haploid) and Diploid mating (hereinafter called Diploid). With colony counter, areas of all yeast colonies obtained by the three mentioned Y1H systems would be calculated. Results showed that the discovery rate of Meiosis is the highest among these three systems. Our laboratory successfully developed a highly efficient Y1H system, which can generate high quality results and consume short time.
Biosynthesis of secondary cell wall plays an important role in wood formation. SND1 (Secondary Wall-Associated NAC Domain 1s) and VND6 (Vascular-Related NAC Domain 6s) family proteins are significant regulators of secondary cell wall differentiation. In previous studies, PtrSND1-A2 and PtrVND6-C1, which belong to SND and VND families of Populus trichocarpa, can interfere in the activation of downstream genes due to intron 2 retention. In this research, we try to explore the evolution of this kind of intron retention in plant species.
Analyzing 10 species, including 2 bryophytes, 1 fern, and 7 angiosperms, we found that neither intron 2 nor 3 retention can’t be observed in only 1 bryophyte among these 11 species. Statistics showed that there was no statistically significant difference in the rate of NAC genes which the specific conditions of intron retention occur between bryophyte-fern group and angiosperm group.
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