| Summary: | 碩士 === 大同大學 === 材料工程學系(所) === 106 === Osteochondral defect indicates the injury to both the articular cartilage and the underlying subchondral bone. In order to repair an osteochondral defect, the needs of the bone, cartilage and the bone-cartilage interface must be jointly taken into consideration. An integrated but heterogeneous composite construct is regarded as promising scaffold for osteochondral graft. In this study, osteochondral substituent is developed and consists of two distinct but integrated layers corresponding to the cartilage and bone components. In the upper layer NAH, a biocompatible in situ forming hydrogel composed of N, O-carboxymethyl chitosan (NOCC) and aldehyde-functionalized hyaluronic acid (AHA) demonstrates similar mechanical, swelling, and lubricating behavior to articular cartilage. In the lower layer CHS, a mineralized sponge scaffold with hydroxyapatite and type I collagen displays porous architecture, osteoconductive, and osteoinductive properties to generate new bone. Two cell types (osteoblast and chondrocyte) were respectively seeded into individual layer of NAH-CHS for in vitro chondrogenesis and osteogenesis investigations. After 21 days culture, the integrity of NAH-CHS could not only be maintained but also showed good cell viability and proliferation over time. It is noteworthy to mention that an integrated bi-phasic NAH-CHS scaffold provides a complete transition between the bone and the cartilage layers without requiring extra manipulative procedures during implantation; thus, simplifying clinical applications. In conclusion, the NAH-CHS can effectively promote osteochondral tissue regeneration in vitro and has great potential for tissue engineering.
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