| Summary: | 碩士 === 臺北醫學大學 === 保健營養學研究所 === 106 === Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) is the major risk factor for the development of gastric ulcer. Lycium barbarum polysaccharides (LBP) have been reported to have antioxidation, immunomodulation, and regeneration actions. C-phycocyanin (C-Pc) is a major biliprotein pigment of Spirulina platensis. C-Pc has a variety of regulatory functions including antioxidation, anti-inflammation, and wound healing properties. The objectives of this study were to investigate the pre-treatment effects of LBP (100,300 and 800 μg/mL) and/or Spirulina extract (30, 250 and 750 μg/mL) for 24 hours on healing of aspirin-induced injury in rat gastric epithelial cell line RGM-1. The results showed that the incubation of 25 mM aspirin for 3 hours significantly increased the activities of NF-κB (p 65) DNA binding and iNOS, and concentrations of MDA, PGE2, mucin 1 and EGF. In addition, 25 mM aspirin significantly decreased SOD activity. The activity of iNOS was significantly inhibited by LBP (300 μg/mL) and Spirulina extract (30 μg/mL). Pretreatment with Spirulina extract (30 μg/mL) also significantly attenuated MDA production. Therefore, the pretreatment of LBP or C-Pc attenuates damage of RGM-1 cells induced by 25 mM aspirin, and returns concentrations of PGE2, MUC1 and EGF closer to normal status.
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