| Summary: | 碩士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 106 === As known that Kallikrein-related peptidases (KLKs) are strongly linked to cancer. In which, KLKs, KLK6 and KLK14 are belong to the downstream gene cluster of androgen receptor (AR) and are regulated by miR378, thereby influence the expression of AKT, which are considered as a useful prognostic indicator of prostate cancer. EPA are proven to be able to reduce harmful immunoreactions. Recent study, indicated that EPA could increase the expression of miR378 to inhibit the growth of tumor cell. MiRNA-378 is one of the non-coding RNA molecules which could control the expression of the protein coding gene. The aim of this study: Firstly, utilizing miRNA-378 to inhibit downstream AR related KLKs thus inhibit the effect of hyperpiasia. Secondly, applying recommended amount of EPA and cell culture media to prostate cancer cell to increase the expression of miR378 and to suppress cancer growth. Thirdly, increase the expression of miRNA-378 to influence the downstream KLKs. The experiment design EPA contained cell culture media to culture prostate cancer cell line, which could stimulate the expression of miRNA-378 western blot to observe protein expression of KLK6 and KLK14. In addition, flow cytometer was used to observe the cell cycle of prostate cancer after applying EPA and miRNA-378. The results showed that after 72 hours of transfecting miRNA-378 of prostate cancer cell, the down expression of KLK6 and KLK14 could be observed, so did the EPA applied cell line. The result inferred suppressing the protein expression in KLK6 and KLK14 could significantly control the deterioration of prostate cancer and might reduce the incurable situations due to drug resistance. Hopefully the correct findings might provide an useful strategy to the clinical therapy in the future.
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