| Summary: | 碩士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 106 === The osteomyelitis caused by implant infection is an urgent issue in clinical, and the prevalence rate locates between 4 to 10% in industrialized countries. It is one of the principal causes of implant failure. Bioactive and multifunctional materials, including bioglass, biometal and biopolymer have been popularly applied in biomedical fields; however, it is still challenge to carry out their effectively antibacterial surface. The purpose of this study is using layer-by-layer technology to develop an antimicrobial coating with outer and inner portions for short/long-term stepwise release of antibacterial agents. It would release antibiotic immediately after implant and antibacterial silver ions in longer period. In this study, polyelectrolyte multilayers (PEMs) coatings comprised of collagen as the cationic layers and γ-poly-glutamic acid as the anionic layers were fabricated onto the 316L stainless steel substrate with a spin coating technique. A chitosan buffer layer was meaningfully designed and allocated in the middle the whole coating. Silver contained 58S bioactive glass (SiO 2 : CaO : P2O 5 = 60:36:4 in wt %) was introduced into the inner γ-poly-glutamic acid layers, and gentamicin outer.
Herein, it is demonstrated that bioactive glass was successfully incorporated into the 20 layered PEMs coating which had a total thickness of about 61.46 ± 2.84 µm (via SEM) and good hydrophilicity with a contact angle of 34.91o. The PEMs coating had a hardness of 0.18 ± 0.014 GPa, Young’s modulus of 3.87 ± 0.32 GPa and roughness of 394.7 nm. The PEMs coating would burst release gentamicin and silver ions in short period, and steadily control release silver ions for long-term antibacterial purpose. The PEMs coating was not only well antibacterial, as seen in the inhibition zone test, but also biocompatible for rat bone marrow mesenchymal stem cells (BMSCs), as seen in the MTT assay. Therefore, we believe that this technology will have great potential in surface modification of orthopedic implants due to its antibacterial, tissue restoration, and osseointegration properties.
|
|---|
