| Summary: | 碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班 === 106 === Electromagnetic Field (EMF) is shown to be able to accelerate bone healing and improve on osteoporosis. However the healing effect of EMF on large size bone fracture (>1cm) is not significant. Tissue engineering uses biocompatible porous scaffolds to fill up large defects, promote cell in-growth and can help reduce bone fracture healing time, and Rapid prototyping (RP) systems produce porous scaffolds with interconnecting pores with evenly distributed cells. This is beneficial for nutritional and metabolic substance transportation to stimulate cell growth, differentiation, and production of extracellular matrix (ECM).
In this study the effects of EMF on cells growing on tissue engineering scaffolds were tested. Osteoblast (7F2) cells were seeded on chitosan scaffold and chitosan scaffold coated magnetic smart carrier, and subjected to EMF (Intensity: 20-30 mT、frequency: 75Hz、Impulse width: 1.3ms) for 2 hours each day for three weeks. On day7、day14、day21, DNA content, alkaline phosphatase activity, calcium content, Type1collagen. And the morphology of the Osteoblast cell was observed by SEM.
After EMF exposure cell viability, proliferation, calcium deposition, ALP activity and DNA content has significantly increased and differentiation performance between experiment and control group. From SEM, typical osteoblast cell morphology was observed on chitosan scaffold(polygonal and spindle-like shape) from the first week .after two to three week of culture ,the cells gradually extended to secrete fibrin and produced large amounts of extracellular matrix(ECM) presents a multi-layer (Multilayer) type. In the material, after the EMF stimulation, the drug release rate have statistical differences, combined with the above results show that containing Fe3O4 carrier scaffold, in the external EMF, can stimulate the growth of bone cells in the future bone repair is a medical application of the value to the material.
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