| Summary: | 碩士 === 亞洲大學 === 食品營養與保健生技學系 === 106 === Psidium guajava, commonly known as guava, is rich in protein, vitamin A, vitamin C, calcium, phosphorus, magnesium and other trace elements, is a very delicious tropical fruit. Guava leaf tea has been used as a hypoglycemic drink as well as a thirst quencher. The guava leaf was extracted with methanol and further partitioned as hexane, ethyl acetate, butanol and aqueous partitions. We set out to examine the cytotoxicity associated with each partition of the guava leaf extract. The aqueous partition is the least cytotoxic partition when compared to other partitions. Previous studies have confirmed that guava leaf extract has a hypoglycemic effect in animal experiments. We would like to know whether the aqueous partition of the guava leaf extract may stimulate glucose uptake in adipocytes. However, 3T3-L1 need to be differentiated before they would respond to insulin stimulation, so we need to optimize 3T3-L1 cell differentiation process before conducting such experiments. We chose to lower fetal bovine serum (FBS) concentration as a starting point of inducing 3T3-L1 cell differentiation, after incubated in differentiation medium (DM)Ⅰfor four days and differentiation mediumⅡfor six days. Glucose uptake test found that 0.1 mg/mL aqueous extract can enhance glucose uptake in adipocytes.
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