Studies on the pharmacological mechanisms of anti-inflammatory and anti-thrombotic effects of Orthosiphon aristatus extract (OAE) on both SVEC4-10 injury and thrombosis in animal model

• Main Authors: Huang,JIAN-GUO, 黃建國
• Other Authors: CHUU,JIUNN-JYE
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/6mhj42

碩士 === 南臺科技大學 === 生物科技系 === 106 === Deep venous thrombosis (DVT) is a condition in which blood coagulate in the deep veins. If it not be untreated, the shedding of thrombus may result in the embolism in the vital organs, and then leads the patient to death. Warfarin, heparin and aspirin are used to prevent the occurrence and recurrence of DVT. However, they are high hemorrhagic and high risk, Therefore, we require more safe and less side-effect treatment. Orthosiphon aristatus (OA) is provided with anti-bacterial, anti-inflammatory effect as a folk traditional herbs. It is used to treat chronic kidney disease and rheumatoid arthritis. Currently, there are many studies about OA extract for antioxidant activity and anti-inflammatory activity of the assessment, but so far there is still no evaluation of the pharmacological activity for the reduction of DVT. It is potentiality that using OA as a drug for the treatment of DVT. In this paper, the in vitro toxicity test of mouse macrophages, mouse fibroblasts and mouse vascular endothelial cells was carried out by using various extracts of Eulaliopsis chinensis. The extracts with LC50> 500 µg / ml were found and their pharmacology was carried out. effect. We used LPS (1 µg/ml) to induce inflammation of mouse macrophages. MWOA and WOA had inhibitory expression of TNF-α and IL-1β mRNA at a dose of 4 µg/ml. And the amount of TNF-α, IL-1β expression in the medium was inhibited. By stimulating SVEC4-10 cell death by administration of H2O2 (100 µM), it was found that MWOA and WOA at a dose of 100 µg/ml protect SVEC4-10 . By administering TNF-α (10 ng/ml) and IL-1β (1 ng/ml) induce SVEC4-10 activation, the results showed that the whisker methanol extract and methanol extract were at a dose of 4 µg /ml has a protein expression amount of inhibiting ICAM-1, VCAM-1 in SVEC4-10 , and an expression amount of IL-6 in the medium. Through the rat thrombus pattern, we found that MWOA and WOA at the dose reached 100 mg/ml, which increased the time of activated coagulation factor (aPTT, PT) and decreased blood. Fibrinogen, reducing the weight of thrombus per unit length, reducing the distribution of red blood cells, white blood cells, and platelets in the blood. For the results of the study, MWOA and WOA have a significant retarding effect on the anti-inflammatory mode and thrombosis in vivo and in vitro, and have potential for future application.