| Summary: | 碩士 === 南臺科技大學 === 生物科技系 === 106 === In recent years, the deterioration of air and the environment has led to moreallergic patients, e.g. allergic rhinitis, asthma and contact dermatitis etc.Recently many studies indicate that the active compounds and pharmacological of C. militaris is even higher and better than that of C. sinensis so that C. sinensis is gradually taken place by C. militaris. Besides, many researchers also apply C. militaris to alleviate the symptoms of various diseases. The results of this study indicated that ethanol extract from silkworm pupa-cultivated C. militaris fruit bodies (CM_EE) could inhibit symptoms of IgE-mediated allergic rhinitis in BALB/c mice. Therefore, we investigated more about the inhibition molecular mechanisms of CM_EE. CM_EE treatment showed the ability to inhibit degranulation in the dose-dependent way. Additionally, CM_EE incubation decreased intracellular calcium influx. Further western immunoblotting results demonstrated that CM_EE incubation could interfere with the activation of Bruton Tyrosine Kinase、Protein Kinase C、p38 mitogen activated protein kinase and 14-3-3γ while promoted the phosphorylation of Eukaryotic translation initiation factor 2 subunit 1 and Peroxisome proliferator-activated receptor γ protein expression. The above results implicated that CM_EE treatment could regulate FcεRI signaling pathway, intracellular calcium level and degranulation to decrease cytokine secretion and thus alleviate allergic rhinitis. In conclusion, CM_EE might be a potential Chinese herbal drug for curing allergy.
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