Analysis of amphetamines in urine by capillary polymeric monolith based microfluidic LC-MS/MS

• Main Authors: LIN, CHIEN-CHENG, 林建成
• Other Authors: FU, MING-REN
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/2j7qry

碩士 === 東吳大學 === 化學系 === 106 === In this study, we used the organic polymer monolithic column (LMA-MAA-EDMA) which was developed by our laboratory combined with the microfluidic liquid chromatography tandem mass spectrometry to detect five amphetamines (amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine) in urine, and the liquid-liquid extraction was used as a method of sample preparation which was easy and simple to reduce the matrix effect caused by urine. Under the optimized conditions, five amphetamines were detected and quantified by using the organic polymer monolithic column (LMA-MAA-EDMA). the linear concentration range was 1 ng/mL to 50 ng/mL, with the coefficients of determination was only greater than 0.99. The relative recovery of the five amphetamines was between 90.2% and 104.3%, and the absolute recovery was between 92.9 % and 122.8 %. The precision and the accuracy of intra-day were between 1.62% and 29.3%, and between 87.9% and 116.78% respectively, the precision and the accuracy of inter-day were between the range of 0.52% and 10.86%, and the range of 92.41% and 114.74 % respectively. Therefore, this method didn’t have the good results compared with the other literatures in the applications of quantitative.