The Effect of Paecilomyces hepiali Mycelium on Alleviation of Neural and Muscle Degeneration

• Main Authors: LIU, PIN-JIE, 劉品杰
• Other Authors: CHIEN, TING-YI
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/8mpn42

碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 106 === Aging is a process for all organisms and appears degeneration in their cellular and biological functions. It is proposed that degenerative diseases involve accumulation of protein aggregates, increased free radical, and environmental toxicology can cause oxidative stress. Mitochondria are the major sites responsible for more than 90% of the ROS generation in the brain and the muscle. The purpose of this study is to use Drosophila and C2C12 mouse myoblast as a degeneracy model to study the anti-degeneration effect of freeze-dried Paecilomyces hepiali mycelia for survival lifespan and western blotting.In the composition analysis, the components were analyzed by high performance liquid chromatography. Total polysaccharide content, total polyphenol content, antioxidant capacity (DPPH scavenging ability, reducing power, ABTS radical scavenging ability) were determined. Results revealed that neurodegeneration hunttington Drosophila model test, CS4 alcohol extract in diet significantly extended the mean lifespan of Drosophila, and improved the climbing ability of young huntington Drosophila. The stress resistance test demonstrated that CS4 alcohol extract could protect effects by hydrogen peroxide-induced oxidative damage but not by paraquat-induced. In C2C12 mouse myoblast as a degeneracy model in vitro test, the CS4 alcohol extract and aqueous extract could significant protective effects by hydrogen peroxide-induced oxidative damage. In the composition analysis, Paecilomyces hepiali mycelia was rich in adenosine and polysaccharides, and alcohol extract was better antioxidant capacity than the aqueous extract. Our results demonstrated that Paecilomyces hepiali mycelia could extend lifespan and increase the resistance to oxidative stress, due to rich polysaccharides, it has a wide range of protection and prevent tissue damage. In the future, we can further investigate the mechanism on alleviation of degeneration.