| Summary: | 碩士 === 靜宜大學 === 食品營養學系 === 106 === Alzheimer's disease (AD) is the most common neurodegenerative diseases of aging. It is characterized by huge β-amyloid (Aβ) accumulation, and neurofibrillary tangles (NFTs) which were form by the phosphorylation of Tau protein in patient's brain. These will induce the rapid degeneration of brain neuron, and then leading the decline of learning and memory. Antrodia comphorata (AC) is a unique fungus in Taiwan. AC contains abundant active components, such as triterpenoids, antroquinonol, polysaccharides and others, and was beneficial on neuroprotection and apoptotic inhibition. However, the effects of AC on learning and memory is still unclear. This study aimed to examine the effect of AC on learning and memory and related mechanism by senescence accelerated mice prone 8 (SAMP8). Three months old male SAMP8 mice were divided into control, and supplemented with low, medium and high dose of AC groups. During the 12 weeks experimental period, body weight, food intake, locomotion, single-trial passive avoidance test and active shuttle avoidance test were performed. After sacrificed, the Aβ accumulation of whole brain, and related regulatory protein expressions of Aβ, Tau, apoptosis of cortex and hippocampus were analyzed. The results showed that SAMP8 mice supplemented with AC could reduce Aβ deposition, and lower the expressions of amyloid precursor protein (APP), β-site amyloid precursor protein cleaving enzyme 1 (BACE-1), phospho-Tau (p-Tau), p53 tumor suppressor protein (p53), and Bcl-2-associated X protein (Bax). AC also increased the phospho-glycogen synthase kinase-3β-ser9 (p-GSK3β-ser9), and pro-cysteine containing asparte-specific protease-3, (procaspase-3) levels, and improved the active and passive avoidance test performances. In summary, AC could enhance the ability of learning and memory, which might due to inhibiting the Aβ-related protein, Tau phosphorylation and apoptosis.
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