| Summary: | 碩士 === 中國文化大學 === 生物科技研究所 === 106 === The aim of this study was to investigate the application of high lactic acid production lactic acid bacteria (Lactobacillus plantarum B2, LB2) and the heterofermentative lactic acid bacteria (LalSil Fresh LB, LAS) with increasing acetic acid production on ensiling of corn forage. The additive effect of condensed molasses fermentation solubles (CMS) inoculation was determined at the same time. Corn Forage (Tainan 19) materials were divided into eight treatment groups including: (1) control, (2) LB2 (1x109 cfu/kg), (3) LAS (3x108 cfu/kg), (4) LB2 (1x109 cfu/kg) + LAS (3x108 cfu/kg), (5) 3.5% CMS, (6) LB2 (1x109 cfu/kg) + 3.5% CMS, (7) LAS (3x108 . cfu/kg) + 3.5% CMS, and (8) LB2 (1x109 cfu/kg) + LAS (3x108 cfu/kg) + 3.5% CMS. The chemical composition and aerobic stability of silage was evaluated after ensiling for 90 days. The in vitro fermentation gas production method was used to evaluate feeding value of silage products. The assay results indicated that the CMS significantly increased lactic acid concentration, reduced the percentage of acetic acid and silage pH at the initial stage of fermentation (p < 0.01). The CMS supplemented with LB2 bacteria improved the quality of silage, but it decreased the aerobic stability (< 24 hr). The CMS + B2 + LAS treatment increased the acetic acid production during the late fermentation period (p < 0.05) and extended the aerobic stability of the silage (> 72 hr). CMS supplementation could improve the gas production of the in vitro fermentation test (p < 0.001), the organic matter digestibility and the microbial protein synthesis efficiency (p < 0.01). Based on all the test outcomes, CMS supplementation might be beneficial to enhance the product stability of corn silage, fermentation ability in the rumen and improve the animal performance.
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