| Summary: | 碩士 === 中國文化大學 === 生物科技研究所 === 106 === Camellia, an evergreen tree, grows in middle and southern regions of China. Its seeds are the raw material of extracting edible oil, which is highly praised because of its unsaturated fatty acids, which had benefit to our body. But after oil extraction, many of the fruit coat usually abandon or be fertilizer. The seeds of plants usually contain various antioxidant factors. Previous studies show that fruit coat had strong antioxidant activity. However, less information is available about the compare of different strain of camellia fruit coat simultaneously, therefore, the present study aimed to evaluate the antioxidant activity of different strain (Camellia oleifera Abel. and Camellia tenuifolia (Hayata) Cohen-Stuart) of the camellia fruit coat. The camellia fruit coat were extracted with ethanol. The antioxidant activity was measured by different models, including DPPH．, ABTS．radical scavenging capacity, H2O2 scavenging capacity and reducing power. In addition, the extracts were measured the total phenol content, total flavonolic content, examined the DNA protection ability and cell viability test. The 70% ethanol extraction of C. tenuifolia fruit coat (crude extract) was separated on a Sephadex LH 20 column chromatography using methanol as mobile phase. The fractions were measured by antioxidant activity. C. tenuifolia had the better DPPH‧ scavenge activity. The C. tenuifolia also had the better ABTS‧ scavenge activity. The total phenol and flavonolic contents distribution in the different strains of Camellia fruit coat was C. tenuifolia more than C. oleifera. The DNA protection experiment showed that all samples had DNA protection abilities with increasing concentration. Furthermore, the fractions through Sephadex LH-20 column chromatography all have good antioxidant activity. In particular, fraction 4 has the best effect. The cytotoxicity test showed that 70% ethanol extraction of Camellia fruit coat were not toxic and inhibit of NO production in LPS-stimulated RAW 264.7 cells. These results demonstrated that the ethanolic extracts of Camellia fruit coat has antioxidant activity, indicating their potential applications are worthy of further investigations.
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