Using Fluidic Sieve Device to Capture Colorectal Cancer Circulating Tumor Cells by Electrochemical Analysis

• Main Authors: Yu-Chuan Liao, 廖宥全
• Other Authors: Jem-Kun Chen
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/wyrv6p

碩士 === 國立臺灣科技大學 === 材料科學與工程系 === 106 === In this study, we use free radical polymerization to synthesize the random copolymer poly(SBMA-co-AA). First we generate the electrode which is nylon sieve with pore size 20 m and it is plated with gold by physical vapor deposition. Then coat copolymer on the electrode by EDC/NHS reaction and modify the surface by using Streptavidin and its specific antibody (Anti-EpCAM conjugated Biotin). The electrode is combined with handmade fluidic device to capture colorectal cancer circulating tumor cell and analyze the quantity by electrochemical methods. We found that the better capture rate is about 52.5 %, the greater anti-fouling property is approximately 99.8 % and the more excellent selectivity occur when the proportion of poly(SBMA-co-AA) is 1:9. Moreover the best efficiency of capture occurs when the flow rate is at 1 mL/hr. The essay shows that electrochemical impedance spectroscopy is highly positive correlation with circulating tumor cell. And it obtains quite low detection limit for 10 circulating tumor cells in 1 mL whole blood. Additionally we apply electrochemical method in cell monitoring and the results indicate that when the cells adhere and grow, the impedance increases. We successfully generate a rapid, convenient and high sensitive fluidic device, which can specifically capture colorectal cancer circulating tumor cells, and quantitatively analyzes by electrochemical methods and cell monitoring.